Journal: Frontiers in Cell and Developmental Biology
Article Title: Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue
doi: 10.3389/fcell.2024.1472338
Figure Lengend Snippet: Gene and protein expression levels of Atp5po, Atp5mg, and Mtatp8. (A–C) : Relative abundance analysis of Atp5po, Atp5mg, and Mtapt8 mRNA in muscle tissues by RT-qPCR; values expressed as mean ± SEM, n = 3, * p < 0.05, ** p < 0.01 for t-test analysis. (D–F) : Relative abundance analysis of ATP5PO, ATP5MG, and MT-ATP8 in muscle tissues by Western blot, values expressed as mean ± SEM, n = 3, * p < 0.05, ** p < 0.01 for t-test analysis. (G) : Bands of Pan-Kla, ATP5PO, ATP5MG, MT-ATP8, and GAPDH proteins.
Article Snippet: The membranes were incubated overnight at 4 °C with the following primary antibodies: MT-ATP8 (1:2,000, Proteintech, China), ATP5MG (1:2,000, Proteintech, China), ATP5PO (1:2000, Proteintech, China), and Anti-L-Lactyl Lysine (1:2,000, PTM Bio, China).
Techniques: Expressing, Quantitative RT-PCR, Western Blot